人碱性成纤维细胞生长因子(bFGF)ELISA试剂盒常见问题解答：
1.问：如果我提取了样本，我是否仍需要遵循试剂盒中说明书给出的建议稀释度？答：提取程序后所需的样品稀释量将受到所用方案中纯化和浓度的影响。稀释量或浓度必须由zui终用户确定。Q:If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?A:The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification and concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.
2.问：我期望得到的分析物的预期浓度是多少？答：给定分析物的量不仅可能因物种而异，也可能因组织和细胞来源而异。这些信息的zui佳来源是通过互联网在多个科学数据库中轻松访问的文献。Q:What is the expected concentration of analyte that I should expect to find?A:The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.
人碱性成纤维细胞生长因子(bFGF)ELISA试剂盒应注意的试验规矩：
1.酶标板从冷藏环境中取出时应康复至室温方可开袋，未用完的酶标板需放入有干燥剂的密封袋中保存。
2.试验操作时需戴手套、穿工作衣，严厉健全和履行消du隔绝原则，各种试验废弃物都应按感染物处理。
3.停止液具有腐蚀性，应避免触摸皮肤和衣物，如不小心触摸请立即用很多自来水冲刷。
4.浓缩洗刷液在低温时简单结晶，使用时需康复至室温以*溶解。
5.洗刷时各孔均需加满液体，避免孔口有游离酶不能洗净。
6.用于检测的样品应坚持新鲜。
7.试验效果的断定有必要以酶标仪读数为准。
8.不同批号试剂组分不得混用。